Page 68 - Instrumental Analysis - Pharm D Clinical- 07-PA403

e. Detector

Function of the detector: monitoring the mobile phase
as it emerges from the column

Characteristics of ideal HPLC detector:
• Adequate sensitivity for the particular task.
• Good stability and reproducibility.
• Wide linear response range.
• A short response time.
• Insensitivity to changes in solvent, flow rate, and

temperature.

Prof. Omayma Abdelrazak Amin 45

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Types of detectors

Bulk property detectors Solute property detectors

• Respond to some physical • Respond to some particular
property of the mobile property of solutes, such as
phase, such as refractive UV absorbance,
index, which is modulated fluorescence
by the presence of solutes
• more selective provide
• universal in application • of high sensitivity

• of poor sensitivity

Prof. Omayma Abdelrazak Amin 46

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