Page 57 - Instrumental Analysis - Pharm D Clinical- 07-PA403

↑ band separation By ↑ α

Done by:
• changing in solvent composition of mob phase
• Changing the composition of stationary phase

Prof. Omayma Abdelrazak Amin 23

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Column Resolution (Rs) (R)

• a quantitative measure of its ability to
separate two analytes

Rs =2[(tR)B – tR)A] / ( WA+WB) 24

Prof. Omayma Abdelrazak Amin

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