Page 52 - Instrumental Analysis - Pharm D Clinical- 07-PA403

• Base Line
• Peak (Peak Base - Peak Area - Peak Width (W) - Peak Height )

• Retention time (tR)
• Dead time (tm)
• Adjusted Retention Time (tR') = tR- tm

Prof. Omayma Abdelrazak Amin 13

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Definition of Terms

• Base Line: That portion of the chromatogram recorded when only the
mobile phase emerges from the column.

• Peak: That portion of the chromatogram recorded while a single
component emerges from the column.

• Peak Base: An interpolation of the base line between the extremities of
the peak. .

• Peak Area: The area enclosed by the peak and the peak base.

• Peak Height: The distance from the peak maximum to the peak base.

• Peak Width t(thWe):inTfhleecmtioangnpiotuindtes of the peak base intercepted by the
tangents to of the peak.

• Retention time (tR): The time between sample injection and. the
appearance of a solute peak at the detector of a chromatographic
column.

• Dead time (tm): The time it takes for an unretained species (mobile
phase) to pass through a column.

• Adjusted Retention Time (tR'): is the time that the solute spent in the
stationary phase. and equals to [ tR- tm].

Prof. Omayma Abdelrazak Amin 14

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