Page 53 - Instrumental Analysis - Pharm D Clinical- 07-PA403

Efficiency of Separation

To achieve successful chromatographic analysis, we
should get :

sharp separated symmetrical chromatographic peaks
within a reasonable run time

↑ rate of band separation
↓ rate of band broadening

Prof. Omayma Abdelrazak Amin 15

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Band Broadening & Column Efficiency

• Column efficiency refers to the amount of band broadening
that occurs when a compound passes through the column

• ↓ Band broadening → ↑ Column efficiency
• This means that band broadening must be limited
• During elution, each solute undergoes thousands of transfers

between stationary and mobile phase
• Movement of solute down the column can occur only while
• the molecule is in the mobile phase

• It is beneficial to measure the efficiency of the column 16

Prof. Omayma Abdelrazak Amin

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