Page 48 - Instrumental Analysis - Pharm D Clinical- 07-PA403
P. 48

Chromatographic analysis is used for
   SEPARATION, quantitation & identification

                      of mixtures

   Strategy of Chromatographic separation

   • The mixture (sample) flows in mobile phase over a
      material (stationary phase)

   • The St. Ph. retains some components more than others
   • So different components flow over the St.Ph. at different

      speeds

   Prof. Omayma Abdelrazak Amin               5

5

   (Partition coefficient)

   Prof. Omayma Abdelrazak Amin               6

6

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