Page 43 - Instrumental Analysis - Pharm D Clinical- 07-PA403
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Spectrofluorimetry
• The simplest heterocyclic compounds, such as pyridine, furan,
thiophene and pyrrole, do not exhibit fluorescence, but fused-ring
structures containing these rings often do.
Non-fluorescent Fluorescent
compounds compounds
13
• Substitution on an aromatic Compound Fluorescence
ring affects the fluorescence Intensity
intensity. The presence of an Benzene 10
electron-donating group, such Toluene 17
as –OH, increases the Phenol 18
fluorescence, while adding an Anisole 20
electron-withdrawing group, Aniline 20
such as –NO2, decreases the Chlorobenzene 7
fluorescence. Bromobenzene 5
Benzoic acid 3
Nitrobenzene 0
14
7
