Page 38 - Instrumental Analysis - Pharm D Clinical- 07-PA403
P. 38

Spectrofluorimetry

               Non-radiative relaxation

1- Vibrational relaxation:
   The molecule can give off some of its energy
   from absorbed light by jumping to a lower
   energy vibrational state. No light is given off.

2- Internal conversion:
   The molecule moves to a lower energy
   electronic state without giving off light. This
   usually happens due to collision with other
   molecules.

3- External conversion:
   The molecule gives off energy to an external
   source, such as by collision with solvent
   molecules.

                                                     3

             Fluorescein  Quinine
Luminol

                             Riboflavin              4

                          2
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