Page 41 - Instrumental Analysis - Pharm D Clinical- 07-PA403

Spectrofluorimetry

Advantages of Fluorometric Analysis

The most attractive features of fluorometry are:
• Sensitivity: concentrations down to 0.001 – 0.1 ppm can be

measured.
• Selectivity: Not all absorbing compounds show fluorescence. This

advantage permits analysis of a fluorescent analyte in complex
mixture of other non-fluorescent compounds.

9

Factors affecting fluorescence

Factors affecting the quantum yield or the ability of the compound to
show fluorescence are:

1- Temperature
2- Solvent (polarity, viscosity, pH)
3- Chemical structure of the compound

10

5