Page 55 - Instrumental Analysis - Pharm D Clinical- 07-PA403
P. 55

Quantitative measure of Column Efficiency

    1) Plate Height (H) (is also known as the Height
       Equivalent of a Theoretical Plate (HETP) ),

    2) Number of Theoretical Plates (N)
        N=16 (tR/W)2
        N=L/H
           Column efficiency is measured by N

       ↑ N →↑ column efficiency

       ↓ HETP (H) → ↑ column efficiency

                      Prof. Omayma Abdelrazak Amin    19

19

To obtain Sharp peak

↑ column efficiency (↓ band broadening)

    by ↑ N (↓H) By ↓ particle size of the packing

    ↓ diameter of column

    ↓ thickness of liq. Film (st ph) (LC)

    Optimizing flow rate of mob. Ph.

                      Prof. Omayma Abdelrazak Amin    20

20

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