Page 60 - Instrumental Analysis - Pharm D Clinical- 07-PA403

Efficiency of Separation

To achieve successful chromatographic analysis, we
should get :

sharp separated symmetrical chromatographic peaks

within a reasonable run time

↓ rate of band broadening ↑ rate of band separation

by ↑ column efficiency ↑ band separation By ↑ α
by ↑ N (↓H) By ↓ particle size of the packing Done by:

↓ diameter of column changing mob. Ph. composition
↓ thickness of liq. Film (st ph) (LC) Changing the composition of st. ph.

Optimizing flow rate of m

Prof. Omayma Abdelrazak Amin 29

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Peak Asymmetry

• Ideal peak has a has a Gaussian shape
• Asymmetric peaks exhibit tailing or fronting
• Asymmetrical peaks can be attributed to a number

of factors including:
- sample overloading

- secondary interactions

Prof. Omayma Abdelrazak Amin 30

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