Page 59 - Instrumental Analysis - Pharm D Clinical- 07-PA403

Prof. Omayma Abdelrazak Amin 27

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Summary

1- Migration Rates of Solutes 2- Band Broadening

k'= (tR – tm)/tm = tR' /tm N = L/H
α = k'B/k'A = (tR')B/(tR')A
N=16 (tR/w)2

Represented in chromatogram by Represented in chromatogram by

tR W

3- Resolution 28

Rs =2[(tR)B – tR)A] / ( WA+WB)

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