Page 25 - MNU-PM502- Pharmaeutical Microbiology Theoritical Book

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

Dry heat Red heat Moist heat
Below 100˚C
- Psteurization
Flaming

At 100˚C

- Boiling water
Incineration - Tyndallization

-

Hot air oven Above 100˚C.
- Steam under pressure
(autocalaving)

A- Dry heat

Utilizes hot air that is free from water vapor and where this moisture plays a minimal
or no role in the process of sterilization

Principle: based on the principle of conduction; that is the heat is absorbed by the
outer surface of an item and then passed onward to the next layer, the entire item

reaches the proper temperature needed to achieve sterilization

Mechanism of destruction of microorganisms

• causing denaturation and lysis of proteins
• drying of cells and can even burn them to ashes(as in incineration)

1. Red heat

• Articles are sterilized by holding them in Bunsen flame till they become red hot.

12