Page 26 - MNU-PM502- Pharmaeutical Microbiology Theoritical Book

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

• This is a simple method but is limited to those articles that can be heated to redness
in flame.

• Examples: bacteriological loops and tips of forceps

2. Flaming

• Passing the article through Bunsen burner without heating to redness.

• Examples: Glass slides, cover slips, Scalpels, Mouth of culture tubes

3. Incineration

• Heating in Furnace at 800˚C to 6500˚C.

• Oxidizes the cell components to ashes or gas.

• results in the loss of the article so, suitable only for those articles that have
to be disposed.

• Due to the high temperatures used, incineration is able to sterilize objects in just a
few seconds to minutes.

• Uses: the preferred method for disposing of

- medical wastes

- animal carcasses contaminated with
infectious agents (Anthrax, Bird flu).

• Hot air Oven
• This is the main method of sterilization by dry heat.

• The device is thermostatically controlled and electrically heated.

• Hot air ovens use high temperatures over several hours to destroy microorganisms
and bacterial spores.

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