Page 102 - MNUmicrobiology practical 2025

General Microbiology & Immunology (PM 401) second level Semester 4 2024/2025

Lowenstein Jensen media
• Composition: It is egg media contains glycerin,

potato starch, asparagine and malachite green

• Color: opaque green media

• Uses: for cultivation of Mycobacterium tuberculosis

Note: Lowenstein Jensen media is enriched &selective media

3. 3. Selective media

This media shows the growth of selective; microbes or desired microorganisms and

inhibits the growth of unwanted microbes. The inhibition occurs by adding bile salts,

antibiotics, dyes, PH adjustments.

Examples: Lowenstein Jensen media

4. 4. Differential media or indicator media

• It distinguishes one microorganism type from another growing on the same media.

• This type of media uses the biochemical characteristics of a microorganism growing

in the presence of specific nutrients or indicators (such as neutral red, phenol red, or

methylene blue) added to the medium to visibly indicate the defining characteristics

of a microorganism

Examples: MacConkey’s media- Simmon’ citrate media- christensen’s urea medium

MacConkey’s media

This media contains lactose and acid indicator

General Microbiology & Immunology (PM 401) Practical note Page 91