Page 105 - MNUmicrobiology practical 2025

General Microbiology & Immunology (PM 401) second level Semester 4 2024/2025

Robertson Cocked meat medium
• Use: Cultivation of anaerobic microorganisms, especially Clostridium species.

• Composition it contains pieces of fat-free minced cooked meat of ox heart and

nutrient broth.

• Principle: Cooked Meat Medium provides a favorable

environment for the growth of anaerobes since the muscle protein

in the heart tissue granules is a source of amino acids and other

nutrients. The muscle tissue also provides reducing substances,

particularly glutathione. The sulfhydryl groups, which exert the

reducing effect, are more available in denatured protein; therefore,

the meat particles are cooked for use in the medium.

6- Media for cultivation of yeast and fungi

Fluid Sabouraud’s media
• Composition: glucose, pepton and water

The pH is 5.6 acidic pH. The acidic pH will favor the growth of fungi.

• Use - Sterility testing of biological specimens

- Cultivation of yeast and fungi

The high concentration of sugar and the acidic pH of the medium permit selectivity
of fungi
• Color: Yellowish brown fluid media

• The media incubated at 25 c for 10 days.,

General Microbiology & Immunology (PM 401) Practical note Page 94