Page 110 - MNUmicrobiology practical 2025

General Microbiology & Immunology (PM 401) second level Semester 4 2024/2025

Learning outcomes

• Evaluate the results of the biochemical tests.

• Judge which tests are appropriate for some bacteria, but not others.

• Formulate how you will organize and address identifying unknown

bacteria based on observations of the patterns in your results.

Biochemical activity of microorganism

• It is study of the chemical changes produced by pure cultures when growing
on various differential media

• It is used in identification and classification of the microorganisms

Biochemical tests of microorganisms
1. Starch hydrolysis

2. Casein hydrolysis
3. Catalase production

4. Oxidase production

5. Sugar fermentation test
6. Nitrate reduction

7. Ammonia production

8. Gelatin hydrolysis
9. H2S production

10. Urease production

11. Hemolysis production
12. IMPiC

General Microbiology & Immunology (PM 401) Practical note Page 99