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#4059

Effect of different altitude on milk fatty acid and conjugated
linoleic acid (CLA) profile

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D Anzhany , Despal , T Toharmat , N Nuraina , A N Hamidah , Noerhayati
1 Study Program Nutrition and Feed Science, Department of Nutrition and Feed
Technology, Faculty of Animal Science, IPB University. Jl. Agatis Kampus IPB
Darmaga 16168, Bogor-Indonesia
2 Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB
University, Jl. Agatis Kampus IPB Darmaga 16168, Bogor-Indonesia
3 Graduate School of Animal Production and Technology Department, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Darmaga 16168, Bogor-
Indonesia

E-mail: despaltk@gmail.com

Abstract. Geographic position and feed quality of farm in highland and lowland particularly in
the tropical region cause the differences in milk quality. One of several factors that caused the
milk quality differences is the fatty acid profile of milk. The objective of this study is to compare
the fatty acid profile of milk produced by the farm in highland and lowland regions. Milk
sampling was done by random purposive methods at two regions that have different altitudes.
Highland region was conducted at Pangalengan sub-district of South Bandung district (984–
1571 masl). Lowland regions were conducted at Tanah Sareal sub-district of Bogor city and
Cibungbulang sub-district of Bogor Regency (200–350 masl). Milk sampling was conducted
with 28 farmers which cows feed botanically diverse forages and commercial mix concentrate
of dairy cows. Milk samples were stored at 4°C until analysis. Prepared milk samples were
stored at – 20°C until analysis for FA profile using Gas Chromatography (GC-7820A/G4350B,
Agilent Technologies) and helium as the carrier gas and flame ionization detector. Individual
fatty acid methyl esters (FAME) were identified by comparison chromatogram peaks of known
standard mixture Supelco 37 Component FAME Mix and standard CLA (O5507, Sigma-
Aldrich). The calculation result of the FA profile was analyzed for normality test and t-test
carried out by SPSS ver.22. Depend on the t-test statistic analysis there is nine significant FA
between both regions. The nine FA consist of one short-chain fatty acid (C6:0), three medium-
chain fatty acids (C10:0, C12:0, and C14:0), and six long-chain fatty acids (C16:0, C18:0,
C18:1-trans9, C18:2-trans, C18:2-cis, and C18:2-CLA). The mean short and medium-chain FA
profile at the highland region higher than the lowland region. The mean long-chain FA profile
at the highland region lower than the lowland region. The conclusion is milk from highland and
lowland regions consists of different FA profiles

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