Page 107 - Practical Handout bio 2 mnu 2025_Neat
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Level two – Semester 4 - PharmD Biochemistry-II PB-403 (Practical course) 2024/2025
Practical work
Assessment of serum creatinine:
Method: colorimetric method.
Principle: colorimetric reaction (Jaffe reaction) of creatinine with
alkaline picrate measured kinetically at 490 nm without any pretreatment
step.
Procedure: as stated in the lab.
Calculations:
Concentration of plasma creatinine =
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