Page 57 - Practical Handout bio 2 mnu 2025_Neat
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Level two – Semester 4 - PharmD      Biochemistry-II PB-403 (Practical course)     2024/2025



                   The Relationship between Absorbance and


                   Concentration



































                     As the sample concentration increases → color intensity increases and

                                                absorbance increases.

                          ✓  The amount of light absorbed can be measured using a

                              colorimeter or spectrophotometer.




































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