Page 58 - Practical Handout bio 2 mnu 2025_Neat
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Level two – Semester 4 - PharmD Biochemistry-II PB-403 (Practical course) 2024/2025
Colorimetric determination of the unknown concentration of
colorless sample:
a) The standard known volume, known concentration.
b) The reagent forms a colored complex with the
sample.
c) Pamphlet provides the work instructions.
N.B: To eliminate the error produced by the reagent, a blank is done.
Blank is to measure the absorbance produced by the reagent.
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