Page 61 - Practical Handout bio 2 mnu 2025_Neat

Level two – Semester 4 - PharmD Biochemistry-II PB-403 (Practical course) 2024/2025

1. What happens to absorbance as the sample concentration increases?

A. Absorbance decreases

B. Absorbance remains constant

C. Absorbance increases

D. Absorbance fluctuates

Answer: C. Absorbance increases

2. Which instrument is used to measure the amount of light absorbed by a

sample?

A. Colorimeter or spectrophotometer

B. Microscope

C. pH meter

D. Centrifuge

Answer: A. Colorimeter or spectrophotometer

3. What is the purpose of a blank in colorimetric determination?

A. To test the sample concentration

B. To measure the absorbance of the reagent only

C. To calibrate the instrument

D. To eliminate the error caused by the standard

Answer: B. To measure the absorbance of the reagent only

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