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SEYYEDI ET AL.                                                                                 |  1053


          affecting gingival health and causing abnormal bleeding such as  The plaque index (PI) (O'Leary et al., 1972), the gingival index
          phenytoin, nifedipine, cyclosporine, enoxaparin, corticosteroids,  (GI) (Löe & Silness, 1963), and the number of decayed, missing, and
          calcium channel blockers, warfarin, and antibiotics over the past  filled teeth at the DMFT level, were recorded by the examiner.
          3 months; not smoking including cigarettes; lack of previously known  Because they were pregnant, caries were only assessed by clinical
          oral disease; informed consent to participate in the study.  examination, and no dental radiographs were taken. Therefore,
                                                              both enamel and dentine caries were recorded together. The
                                                              number of decayed and filled teeth was separately recorded to
          2.3  | Exclusion criteria                           determine whether the pregnant women had referred for neces-
                                                              sary dental treatments prior to pregnancy. A DMFT score was then
          Employment in professions related to dentistry; failure to complete  calculated.
          the questionnaire completely; reluctance to participate in an oral  The O'Leary et al. (1972) method was utilized for the
          examination; history of pregnancy complications, such as severe  evaluation of dental plaque. The participant first rinsed her mouth
          bleeding or pre‐eclampsia.                          with water to remove any debris. Then, the subject was asked to
                                                              use plaque‐disclosing tablets and rinse her mouth again. For each
                                                              tooth, buccal, lingual, mesial, and distal surfaces were assessed.
          2.4  | Data collection                              Finally, the number of colored surfaces compared to the total area
                                                              was estimated as a percentage (O'Leary et al., 1972; Tibério
          First, all selected participants completed a three‐part questionnaire  et al., 2017).
          after signing the informed consent form. The first part of the  The GI was assessed using the Löe and Silness method. The GI
          questionnaire included demographic characteristics such as age,  score of each surface of the tooth was defined as:
          educational level, occupation, month of pregnancy, pregnancy  Normal gingiva scored as 0; mild inflammation: a slight change in
          characteristics, abortion history, number of previous pregnancies,  color and edema without bleeding during probing scored as 1;
          and medical and medication history. The next two sections included  moderate inflammation: existence of redness, edema, and bleeding
          questions that involved participants' knowledge and attitude and oral  during probing scored as 2; and severe inflammation: definite redness
          health practice during pregnancy.                   and edema, spontaneous wound, and bleeding scored as 3.
             This specially designed questionnaire was based on those used in  The score for each tooth was estimated by calculating the mean
          previous studies and credible sources. The validity of the question-  score of its four surfaces. According to the standard, if the GI of each   20574347, 2023, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1002/cre2.804 by Readcube (Labtiva Inc.), Wiley Online Library on [01/02/2025]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicabl
          naire was confirmed using the content validation method and after  tooth was from 0.1 to 1, it was classified as mild inflammation, from
          seeking the opinions of six oral medicine specialists. To verify the  1.1 to 2: defined as moderate inflammation, and from 2.1 to 3,
          reliability of the questionnaire, it was evaluated by retesting. The  considered as severe inflammation.
          questionnaire was completed for a second time by 15 pregnant  The resulting data were collected and analyzed using SPSS
          women after an interval of 21 days. The correlation coefficient  (version.18) software with paired t test and Pearson's correlation
          between the responses confirmed the reliability of the questionnaire  coefficient.
          (r = .86 for the attitude section and r = .75 for the knowledge section).
             The knowledge section included 10 questions which were
          designed as three‐choice answers including “yes,”“no,” and “Ido  3 |  RESULTS
          not know.” Each correct answer received a score of +1, every wrong
          answer a score of −1, and every neutral answer a score of zero. The  3.1 |  Characteristics of the participants
          highest score in this section was +10 and the lowest score was −10.
             The attitude assessment section consisted of eight statements,  The results regarding the demographic characteristics of research
          which included four options based on the Likert scale: “I completely  subjects including age, gestational week, number of previous
          disagree,”“I disagree,”“I agree,” and “I completely agree.” The  pregnancies, history of abortion, previous pregnancy problems, as
          attitude section was scored between −16 and +16.    well as dental problems in previous pregnancies can be seen inTable 1.
             If the subjects were illiterate, in order not to bias the answers,  The mean age of the pregnant women was 29.11 ± 6.80 years and the
          the researcher would explain the questions to them, without  level of education in 56 (58%) of the subjects was below a high school
          changing her facial expression.                     diploma. Since pregnant women visited this health center in the
                                                              morning, all of them were housewives.
                                                                 In terms of oral hygiene, out of the 96 participants, 83 (87%)
          2.5  | Oral examination                             reported brushing their teeth. Among them, 5 (5%) said they brushed
                                                              three times a day, 11 (12%) twice a day, 62 (65%) reported brushing
          In the next step, an oral examination was performed by a specially  their teeth only once a day, and 5 (5%) rarely used a toothbrush. A
          trained dental student under the supervision of an oral medicine  few reported using dental floss or a mouthwash and eight did not use
          specialist, using a disposable dental probe and mirror.  any product for oral care.
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