Page 354 - 2014 Printable Abstract Book
P. 354
PS 7 LATE BREAKING
(PS7-1) Laser accelerated ultra-high dose rate proton and carbon ion induced DNA damage and repair
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2
1
kinetics in human AG01522 cells. Pankaj Chaudhary, PhD ; Fiona Hanton, MSc ; Domenico Doria, PhD ;
4
3
1
2
Deborah Gwynne, M.Sc. ; Thomas Marshall, M.Sci. ; Kealan Naughton, MSc ; Lorenzo Romagnani, PhD ;
Satya Kar, PhD ; Giuseppe Schettino, PhD ; Marco Borghesi ; and Kevin M. Prise, PhD , Centre for Cancer
3
5
2
1
2
1
Research and Cell Biology, Belfast, United Kingdom ; Centre of Plasma Physics, Belfast, United Kingdom ;
4
3
Centre for Plasma Physics, Belfast, United Kingdom ; Laboratoire LULI Ecole Polytecnique, Cedex, France ;
5
and National Physical Laboratory, London, United Kingdom
Hadrontherapy is being developed worldwide as an advanced and effective modality for tumor
therapy. However, significant setup and operational costs limit the numbers of patients that can access
the potential benefits. Significant advances in Laser technologies have made it possible to generate high
energy and ultrahigh dose rate ion beams at potentially considerably lower operational costs than
conventional particle accelerators. An understanding of the radiobiological effects at ultrahigh dose rate
in picosecond pulses generated by the Laser accelerated ions on the human cells is important for
development and further advancement of this technology towards clinical applications.
10
Laser accelerated 10-20 MeV protons and 8 MeV/n carbon ions at an ultrahigh dose rate of 10 Gy/sec,
were generated using a Titanium: Sapphire Laser system at Astra Gemini in the Rutherford Appleton
Laboratory, Oxford, UK. Dosimetry was performed using EBT2 gafchromic film and the ion beams profile
was characterized using a Thomson Parabola ion spectrometer. Normal human skin fibroblasts (AG01522
cells) monolayers grown in custom made stainless steel, on 3 μm thin Mylar dishes were irradiated and
DNA damage and repair kinetics were quantified using 53BP1 foci formation assay up to 24 hours after
irradiation. Data from our experiments suggest that Laser accelerated proton-induced DNA damage was
substantially repaired upto 24 hours as seen by reduction in the average number of 53BP1 foci per
nucleus, however the DSB damage caused by carbon ions persisted up to 24 hours post irradiation
indicating increased complexity of the damage and following the trend reported for conventional
cyclotron accelerator generated protons and carbon ions. We report here for the first time measurements
10
of DNA damage with pulsed carbon ions at ultrahigh dose rate (10 Gy/s). Further refinements in the
beam delivery and energy upgrades are being developed for future translation to relevant clinical settings.
(PS7-2) Lifetime cancer risks attributable to occupational radiation exposure of NDT workers in South
2
2
1
1
Korea. Songwon Seo, M.S. ; Eun-Kyeong Moon, M.S. ; Won Jin Lee, MD, PhD ; Dal Nim Lee, M.S. ; Min-
1
1
1
1
Jung Kim, PhD ; Ki Moon Seong, PhD ; Sunhoo Park, MD, PhD ; Seung-Sook Lee, MD, PhD ; and Young
1
Woo Jin, MD, Ph.D , National Radiation Emergency Medical Center, Korea Institute of Radiological &
1
Medical Sciences, Seoul, Korea, Republic of and Department of Preventive Medicine, College of Medicine,
2
Korea University, Seoul, Korea, Republic of
Radiation exposure of most radiation workers in South Korea is well monitored with an average
exposure of 1.59 mSv/yr in 2012. Although an average exposure of non-destructive testing (NDT) workers
is tolerable with 3.4 mSv/year, about 9% and 2% of the workers were observed with more than 10 and 20
mSV/yr, respectively, which is relatively higher than other radiation-related occupations. The purpose of
the study is to estimate lifetime attributable risk (LAR) of cancer incidence for occupationally exposure
from ages 20 to 55 with the selected doses (mSv/yr) of 10, 20 and 30 on the distribution of radiation
352 | P a g e
(PS7-1) Laser accelerated ultra-high dose rate proton and carbon ion induced DNA damage and repair
2
2
1
kinetics in human AG01522 cells. Pankaj Chaudhary, PhD ; Fiona Hanton, MSc ; Domenico Doria, PhD ;
4
3
1
2
Deborah Gwynne, M.Sc. ; Thomas Marshall, M.Sci. ; Kealan Naughton, MSc ; Lorenzo Romagnani, PhD ;
Satya Kar, PhD ; Giuseppe Schettino, PhD ; Marco Borghesi ; and Kevin M. Prise, PhD , Centre for Cancer
3
5
2
1
2
1
Research and Cell Biology, Belfast, United Kingdom ; Centre of Plasma Physics, Belfast, United Kingdom ;
4
3
Centre for Plasma Physics, Belfast, United Kingdom ; Laboratoire LULI Ecole Polytecnique, Cedex, France ;
5
and National Physical Laboratory, London, United Kingdom
Hadrontherapy is being developed worldwide as an advanced and effective modality for tumor
therapy. However, significant setup and operational costs limit the numbers of patients that can access
the potential benefits. Significant advances in Laser technologies have made it possible to generate high
energy and ultrahigh dose rate ion beams at potentially considerably lower operational costs than
conventional particle accelerators. An understanding of the radiobiological effects at ultrahigh dose rate
in picosecond pulses generated by the Laser accelerated ions on the human cells is important for
development and further advancement of this technology towards clinical applications.
10
Laser accelerated 10-20 MeV protons and 8 MeV/n carbon ions at an ultrahigh dose rate of 10 Gy/sec,
were generated using a Titanium: Sapphire Laser system at Astra Gemini in the Rutherford Appleton
Laboratory, Oxford, UK. Dosimetry was performed using EBT2 gafchromic film and the ion beams profile
was characterized using a Thomson Parabola ion spectrometer. Normal human skin fibroblasts (AG01522
cells) monolayers grown in custom made stainless steel, on 3 μm thin Mylar dishes were irradiated and
DNA damage and repair kinetics were quantified using 53BP1 foci formation assay up to 24 hours after
irradiation. Data from our experiments suggest that Laser accelerated proton-induced DNA damage was
substantially repaired upto 24 hours as seen by reduction in the average number of 53BP1 foci per
nucleus, however the DSB damage caused by carbon ions persisted up to 24 hours post irradiation
indicating increased complexity of the damage and following the trend reported for conventional
cyclotron accelerator generated protons and carbon ions. We report here for the first time measurements
10
of DNA damage with pulsed carbon ions at ultrahigh dose rate (10 Gy/s). Further refinements in the
beam delivery and energy upgrades are being developed for future translation to relevant clinical settings.
(PS7-2) Lifetime cancer risks attributable to occupational radiation exposure of NDT workers in South
2
2
1
1
Korea. Songwon Seo, M.S. ; Eun-Kyeong Moon, M.S. ; Won Jin Lee, MD, PhD ; Dal Nim Lee, M.S. ; Min-
1
1
1
1
Jung Kim, PhD ; Ki Moon Seong, PhD ; Sunhoo Park, MD, PhD ; Seung-Sook Lee, MD, PhD ; and Young
1
Woo Jin, MD, Ph.D , National Radiation Emergency Medical Center, Korea Institute of Radiological &
1
Medical Sciences, Seoul, Korea, Republic of and Department of Preventive Medicine, College of Medicine,
2
Korea University, Seoul, Korea, Republic of
Radiation exposure of most radiation workers in South Korea is well monitored with an average
exposure of 1.59 mSv/yr in 2012. Although an average exposure of non-destructive testing (NDT) workers
is tolerable with 3.4 mSv/year, about 9% and 2% of the workers were observed with more than 10 and 20
mSV/yr, respectively, which is relatively higher than other radiation-related occupations. The purpose of
the study is to estimate lifetime attributable risk (LAR) of cancer incidence for occupationally exposure
from ages 20 to 55 with the selected doses (mSv/yr) of 10, 20 and 30 on the distribution of radiation
352 | P a g e
