Page 359 - 2014 Printable Abstract Book
P. 359
antibody binding or other immunological assays. Finally, other dominant radiation products were
detected that could be potential targets for a biodosimetric assay, namely specific protein-protein cross
linking reactions that formed at low doses (<10 Gy) and were readily detected using simple time-of-flight
(TOF) mass spectroscopy.
(PS7-11) Metabolic analysis of grade IV glioblastoma by induced metabolic bioluminscence imaging
2
1
1
1
1
2
(imBI). Kristina Goetze ; Nadine Voelxen ; Jacob Boettcher ; Stefan Walenta ; Ella L. Kim ; Alf Giese ; and
1
Wolfgang Mueller-Klieser , Institute of Pathophysiology, University Medical Center Mainz, Mainz,
2
Germany and Department of Neurosurgery, University Medical Center Mainz, Mainz, Germany
1
Chronic accumulation of lactate in tumor tissue is associated with an increased malignancy and
radio-resistance. Additionally, a new possible prognostic marker, especially for glioblastomas, seems to
emerge in D-2-hydroxyglutarate (2HG), a metabolic product of the conversion of α-ketoglutarate by a
mutant isocitratedehydrogenase (IDH). The aim of the study was the metabolic analysis of primary or
secondary glioblastoma (WHO grade IV) using induced metabolic bioluminescence imaging (imBI),
correlating lactate content with the expression of monocarboxylate transporters (MCT) and patient
survival. In a parallel setup we aim to establish the imBI measurement of 2HG. Cryobiopsies of 22
glioblastoma patients were used to determine ATP, lactate and glucose concentrations using imBI. In
parallel sections of these biopsies the expressions of monocarboxylate transporters (MCT1 and MCT4)
were analyzed using immunofluorescence staining. In glioblastoma tissue high lactate concentrations with
only a small number of lower lactate level samples were detected. The comparison of lactate content with
MCT expression revealed a significant correlation of lactate concentrations and MCT1 but no correlation
with MCT4. Furthermore, a significant, yet relatively small difference in tumor lactate content of patients
that survived less than 17 month compared to patients that survived for longer periods was detected. In
conclusion, imBI measurements of glioblastomas revealed high lactate concentrations that significantly
correlated with MCT1 expression and with patient overall survival. But the results so far do not suffice for
an improvement of the current prognostic parameters for glioma patients. Establishing a locoregional
measurement of 2HG using imBI might allow for a better prediction of the clinical outcome. Supported by
the BMBF: ISIMEP, 02NUK016A
(PS7-12) Modeling the Trajectory of Psycho-Social Anxiety in Ukraine following the Chornobyl Nuclear
2
1
3
2
Accident. Robert A. Yaffee, PhD ; Thomas B. Borak ; RoseMarie Perez-Foster ; Remi Frazier ; Mariya
5
6
4
Burdina ; Gleb Prib ; and Victor Chtenguelov , Silver School of Social Work, New York University, New
2
1
York, NY ; Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO ;
3
Natural Hazards Center, University of Colorado, Boulder, CO ; Department of Economics, University of
4
Colorado, Boulder, CO ; Institute of Professional Training of Public Service of Employment of Ukraine, Kiev,
5
Ukraine ; and Department of Social Work and Applied Psychology, Academy of Labor and Social Relations,
Kiev, Ukraine
6
Our objectives were to examine predictive parameters of principal psychological impacts,
resulting from the Chornobyl accident, on residents living in Kiev and Zhitomyr oblasts. In this
presentation, we tested predictors of psycho-social anxiety based on estimated radiological dose received
from radioactivity released during the accident and the perception of health risk associated with this
357 | P a g e
detected that could be potential targets for a biodosimetric assay, namely specific protein-protein cross
linking reactions that formed at low doses (<10 Gy) and were readily detected using simple time-of-flight
(TOF) mass spectroscopy.
(PS7-11) Metabolic analysis of grade IV glioblastoma by induced metabolic bioluminscence imaging
2
1
1
1
1
2
(imBI). Kristina Goetze ; Nadine Voelxen ; Jacob Boettcher ; Stefan Walenta ; Ella L. Kim ; Alf Giese ; and
1
Wolfgang Mueller-Klieser , Institute of Pathophysiology, University Medical Center Mainz, Mainz,
2
Germany and Department of Neurosurgery, University Medical Center Mainz, Mainz, Germany
1
Chronic accumulation of lactate in tumor tissue is associated with an increased malignancy and
radio-resistance. Additionally, a new possible prognostic marker, especially for glioblastomas, seems to
emerge in D-2-hydroxyglutarate (2HG), a metabolic product of the conversion of α-ketoglutarate by a
mutant isocitratedehydrogenase (IDH). The aim of the study was the metabolic analysis of primary or
secondary glioblastoma (WHO grade IV) using induced metabolic bioluminescence imaging (imBI),
correlating lactate content with the expression of monocarboxylate transporters (MCT) and patient
survival. In a parallel setup we aim to establish the imBI measurement of 2HG. Cryobiopsies of 22
glioblastoma patients were used to determine ATP, lactate and glucose concentrations using imBI. In
parallel sections of these biopsies the expressions of monocarboxylate transporters (MCT1 and MCT4)
were analyzed using immunofluorescence staining. In glioblastoma tissue high lactate concentrations with
only a small number of lower lactate level samples were detected. The comparison of lactate content with
MCT expression revealed a significant correlation of lactate concentrations and MCT1 but no correlation
with MCT4. Furthermore, a significant, yet relatively small difference in tumor lactate content of patients
that survived less than 17 month compared to patients that survived for longer periods was detected. In
conclusion, imBI measurements of glioblastomas revealed high lactate concentrations that significantly
correlated with MCT1 expression and with patient overall survival. But the results so far do not suffice for
an improvement of the current prognostic parameters for glioma patients. Establishing a locoregional
measurement of 2HG using imBI might allow for a better prediction of the clinical outcome. Supported by
the BMBF: ISIMEP, 02NUK016A
(PS7-12) Modeling the Trajectory of Psycho-Social Anxiety in Ukraine following the Chornobyl Nuclear
2
1
3
2
Accident. Robert A. Yaffee, PhD ; Thomas B. Borak ; RoseMarie Perez-Foster ; Remi Frazier ; Mariya
5
6
4
Burdina ; Gleb Prib ; and Victor Chtenguelov , Silver School of Social Work, New York University, New
2
1
York, NY ; Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO ;
3
Natural Hazards Center, University of Colorado, Boulder, CO ; Department of Economics, University of
4
Colorado, Boulder, CO ; Institute of Professional Training of Public Service of Employment of Ukraine, Kiev,
5
Ukraine ; and Department of Social Work and Applied Psychology, Academy of Labor and Social Relations,
Kiev, Ukraine
6
Our objectives were to examine predictive parameters of principal psychological impacts,
resulting from the Chornobyl accident, on residents living in Kiev and Zhitomyr oblasts. In this
presentation, we tested predictors of psycho-social anxiety based on estimated radiological dose received
from radioactivity released during the accident and the perception of health risk associated with this
357 | P a g e
