Page 361 - 2014 Printable Abstract Book
P. 361
(PS7-14) Radiation induces radiobioluminescence in fish. Rao V. Papineni, Ph.D 1; 2 ; PACT & Health,
1
2
Branford, CT and Kansas University Medical Center (Adjunct), Kansas, KS
Fish would be an ideal biological system to report nuclear accidents and contamination of water
bodies. Here we demonstrate incorporating optical imaging in radiation therapy, whole body irradiation
of fish leads to release of endogenous bioluminescence. This is distinct from the cherenkov radiation. Live
fish were subjected to 4 Gy X-ray irradiation doses using commercially available X-RAD 320 irradiator
(1Gy/min; F2 beam hardening filter 1.5mm Al, 0.25mm Cu, 0.75mm Sn; Precision X-ray inc, USA). The
endogenous bioluminescence in response to irradiation, described here as radiobioluminescence, was
captured using cooled CCD camera from live subjects. Significant increase in the amounts of photons
released as bioluminescence was detected. The radiobioluminescence was present all over the body with
negligible amounts in the caudal fin region. The radiobioluminescence lifetime measurements in different
animal models and its relationship with the nutrients and metabolites, topical photosensitizers during the
course of radiation therapy are elaborated. Apart from the potential use of such technology in nuclear
preparedness, these molecular imaging methodologies and modalities described will be useful tools in
radiation treatment design and process.
(PS7-15) Study of radiation related circulatory diseases using an animal model: Introducing spontaneous
1
hypertensive rat (SHR) and stroke prone SHR (SHRSP) as animal models. Norio Takahashi, PhD ; Hideko
2
2
4
3
Murakami, MD ; Waka Ohishi ; Munechika Misumi, PhD ; Yoichiro Kusunoki, PhD ; Akiko Nagamachi,
6
5
5
6
PhD ; Toshiya Inaba, MD,PhD ; Yoichi Oghiso, PhD ; Satoshi Tanaka, DVM, PhD ; Ignacia Braga-Tanaka,
6
2
DVM, PhD ; and Yasuharu Niwa, PhD ; Consultant, Radiation Effects Research Foundation, Hiroshima,
1
Japan ; Department of Radiobiology/Molecular Epidemiology, Radiation Effects Research Foundation,
2
Hiroshima, Japan ; Department of Clinical Studies, Radiation Effects Research Foundation, Hiroshima,
4
3
Japan ; Department of Statistics, Radiation Effects Research Foundation, Hiroshima, Japan ; Research
5
Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan ; and Department of
Radiobiology, Institute for Environmental Sciences, Rokkasho,Aomori, Japan
6
Data from atomic-bomb survivors indicate radiation-associated risk for circulatory diseases (CDs).
The issues received keen attention in the field of radiation protection, radiation biology and cardiology.
We conduct animal model studies in order to assess the relationship between CDs and radiation exposure
and to obtain information about biological mechanisms. We selected two inbred strains, SHR and SHRSP.
Before starting the study, we conducted a preliminary study to evaluate the feasibility of using each strain
as an animal model of radiation effects on CD. In studies using SHRSP, the life span of rats irradiated with
1Gy or more was significantly shorter than unirradiated rats, and the perivascular damage in irradiated
rats was more advanced than that observed in the organs of unirradiated rats, thus confirming that the
SHRSP is an appropriate model. A study of SHRSP rats given exposure of 0, 0.25, 0.5, 0.75 and 1Gy is
ongoing. However, we learned that SHRSP strain was not appropriate for assessing evidence of radiation-
associated risk for hypertension, since measuring blood pressure after observing initial symptoms of
stroke can lead to further stroke symptoms. Thus, we introduced SHR rats, since SHR rats do not show
stroke symptoms. In a preliminary study to evaluate the feasibility of the SHR strain, we successively
measured blood pressure until 25 weeks of age. The systolic blood pressure level of irradiated rats tended
to be higher than that of unirradiated ones. The body weight of irradiated rats was significantly lower than
that of unirradiated ones. These data revealed that SHR was suitable model animals for the study of blood
359 | P a g e
1
2
Branford, CT and Kansas University Medical Center (Adjunct), Kansas, KS
Fish would be an ideal biological system to report nuclear accidents and contamination of water
bodies. Here we demonstrate incorporating optical imaging in radiation therapy, whole body irradiation
of fish leads to release of endogenous bioluminescence. This is distinct from the cherenkov radiation. Live
fish were subjected to 4 Gy X-ray irradiation doses using commercially available X-RAD 320 irradiator
(1Gy/min; F2 beam hardening filter 1.5mm Al, 0.25mm Cu, 0.75mm Sn; Precision X-ray inc, USA). The
endogenous bioluminescence in response to irradiation, described here as radiobioluminescence, was
captured using cooled CCD camera from live subjects. Significant increase in the amounts of photons
released as bioluminescence was detected. The radiobioluminescence was present all over the body with
negligible amounts in the caudal fin region. The radiobioluminescence lifetime measurements in different
animal models and its relationship with the nutrients and metabolites, topical photosensitizers during the
course of radiation therapy are elaborated. Apart from the potential use of such technology in nuclear
preparedness, these molecular imaging methodologies and modalities described will be useful tools in
radiation treatment design and process.
(PS7-15) Study of radiation related circulatory diseases using an animal model: Introducing spontaneous
1
hypertensive rat (SHR) and stroke prone SHR (SHRSP) as animal models. Norio Takahashi, PhD ; Hideko
2
2
4
3
Murakami, MD ; Waka Ohishi ; Munechika Misumi, PhD ; Yoichiro Kusunoki, PhD ; Akiko Nagamachi,
6
5
5
6
PhD ; Toshiya Inaba, MD,PhD ; Yoichi Oghiso, PhD ; Satoshi Tanaka, DVM, PhD ; Ignacia Braga-Tanaka,
6
2
DVM, PhD ; and Yasuharu Niwa, PhD ; Consultant, Radiation Effects Research Foundation, Hiroshima,
1
Japan ; Department of Radiobiology/Molecular Epidemiology, Radiation Effects Research Foundation,
2
Hiroshima, Japan ; Department of Clinical Studies, Radiation Effects Research Foundation, Hiroshima,
4
3
Japan ; Department of Statistics, Radiation Effects Research Foundation, Hiroshima, Japan ; Research
5
Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan ; and Department of
Radiobiology, Institute for Environmental Sciences, Rokkasho,Aomori, Japan
6
Data from atomic-bomb survivors indicate radiation-associated risk for circulatory diseases (CDs).
The issues received keen attention in the field of radiation protection, radiation biology and cardiology.
We conduct animal model studies in order to assess the relationship between CDs and radiation exposure
and to obtain information about biological mechanisms. We selected two inbred strains, SHR and SHRSP.
Before starting the study, we conducted a preliminary study to evaluate the feasibility of using each strain
as an animal model of radiation effects on CD. In studies using SHRSP, the life span of rats irradiated with
1Gy or more was significantly shorter than unirradiated rats, and the perivascular damage in irradiated
rats was more advanced than that observed in the organs of unirradiated rats, thus confirming that the
SHRSP is an appropriate model. A study of SHRSP rats given exposure of 0, 0.25, 0.5, 0.75 and 1Gy is
ongoing. However, we learned that SHRSP strain was not appropriate for assessing evidence of radiation-
associated risk for hypertension, since measuring blood pressure after observing initial symptoms of
stroke can lead to further stroke symptoms. Thus, we introduced SHR rats, since SHR rats do not show
stroke symptoms. In a preliminary study to evaluate the feasibility of the SHR strain, we successively
measured blood pressure until 25 weeks of age. The systolic blood pressure level of irradiated rats tended
to be higher than that of unirradiated ones. The body weight of irradiated rats was significantly lower than
that of unirradiated ones. These data revealed that SHR was suitable model animals for the study of blood
359 | P a g e
