Page 143 - PG 504-theoretical notes-phyto-1-2024-2025..

Clinical pharmacy PharmD program Third level Phytochemistry-1 (PG-504)

▪ The enzyme amygdalase is specific for the glycoside amygdalin.

▪ The enzyme myrosin (or myrosinase) is specific for thio-glucosides : e.g.
sinigrin and sinalbin

▪ The enzyme prunase is the specific hydrolyzing enzyme for the glycoside
prunasin and other (1ry) glycosides

Distinction of the Different Glycosidic Linkages

- 1 Using universal enzymes :

Enzymatic hydrolysis is specific for each glycoside (or a type of glycosides)

Enzyme Substrate (Glycoside type)

Emulsin (a mixture of -enzymes hydrolyze most -glycosidic linkages

Maltase and invertase hydrolyzing -glycosides only

Quantitative estimation of glycosides:

• For sugar:

Quantitative assays (copper reduction, iodometry, enzymatic)

• For aglycone:

Quantitative assays (depending on the aglycone chemical nature)

•Bioassay: by testing the biological action of the glycoside.

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