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วารสารเทคโนโลยีชีวภาพการผลิตปศุสัตว์

the correct meat species as label. This demonstrated that regulation of meat production could be

strengthened in purpose of food safety, religious, and to prevent illegal fraud of meat product. The

application of this method is not only for human consumption such as Halal authentication but also

benefit for tracing animal feed adulteration. However, the analytical conditions might be improved
for quantitative differentiation in fewer amounts of DNA as well as being able to identification more

animal species.

Conclusion

Using multiplex PCR with specific primer design based on mt cyt b gene and appropriated

reaction condition, meat from 6 species could be identified at the same time. Therefore, it should be
a potential tool for routine analysis of meat species identification since it could be performed rapidly

and quite sensitive.

References

Anonymous. The National Center of Biotechnology Information (NCBI). Published mitochondrial cyt

b gene sequences. Available source:http://www.ncbi.nlm.nih.gov, Jan 12, 2016.
Anonymous. Primer3plus. Primer design program. Available source:http://www.bioinformatics.nl/cgi-

bin/primer3plus/primer3plus.cgi, Mar 10, 2016.

Anonymous. The sequencing information of GenBank (NCBI) using program. The BLAST Sequence

Analysis. Available source:https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch

#, Jul 5, 2016.

Bottero, M.T., A. Dalmasso, D. Nucera, R.M. Turi, S. Rosati, S. Squadrone, M. Goria and T. Civera.

2003. Development of a PCR assay for the detection of animal tissues in ruminant feeds.
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Cheunsuk, S., N. Sarataphan and W. Tularuksa. 2016. Use of Cytochrome b Gene Variability in

Ruminant Species Differentiation. Proceeding in the National Livestock Conference. August

23-25, 2016.

Dalmasso, A., E. Fontanella, P. Piatti, T. Civera, S. Rosati and M.T. Bottero. 2004. A multiplex PCR

assay for the identification of animal species in feedstuffs. Molecular and Cellular Probes.

18:81-87.

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