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           Figure 2.  Biochemical profile of plasma samples from control (PBS) and treated (goat milk exosomes) mice, collected 24 h after dose administration.
           Asterisks (*) indicate statistically significant differences (p < 0.05), observed only for triglycerides.

           4000 relative fluorescent units in both cases. Additionally, the   population could be considered 100% positive for the labeling,
                                                                                              3
                                                                                           1
           fluorescence of the resulting probes was evaluated by flow   as the control’s autofluorescence of 10 –10  relative fluorescent
                                                          5
           cytometry (Figure  3), showing an intense peak between 10    units did not overlap with that of the labeled exosomes in either
                6
           and 10  for both Exo-BDP and Exo-SCy5. The whole analyzed   wavelength.







































           Figure 3.  Physicochemical characterization of fluorescent milk exosomes, including NTA analysis, TEM images (100 000×), and flow cytometry for
           a) Exo-BDP and b) Exo-SCy5.



           Small 2021, 2105421             2105421  (4 of 12)              © 2021 The Authors. Small published by Wiley-VCH GmbH
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