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Figure 5. Assessment of exosome uptake by RAW 264.7 macrophages. a) Confocal images of macrophages after 24 h of exposure to different concentra-
tions of exosomes. Blue (DAPI), red (phalloidin) and green (Exo-BDP). b) Quantification of fluorescent exosome uptake by RAW 264.7 cells in confocal
imaging. Statistical analysis by ANOVA and post-hoc Student-Newman-Keuls multiple comparisons test.
Figure 6. In vitro evaluation of Exo-BDP uptake in macrophage populations. a) Flow cytometry of M0, M1, and M2 populations after 1, 4, and 24 h of
−1
incubation with 0.5 µg mL Exo-BDP. b) Quantification of fluorescence intensity by flow cytometry. Statistical analysis by two-way ANOVA and Tukey’s
multiple comparisons test; p values below 0.05: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. c) Confocal images of Exo-BDP uptake in M0,
M(IFN-γ+LPS)/M1, and M(IL-4)/M2 populations. IFN-γ, interferon gamma; LPS, lipopolysaccharide; IL-4, interleukin-4.
Small 2021, 2105421 2105421 (6 of 12) © 2021 The Authors. Small published by Wiley-VCH GmbH
