Page 104 - Catalogue - cuvettes and solid-state calibration standards

Resolution of spectral bandwidth of UV/VIS spectrometers

Part Number Description Price
FF-SRM-TiH Toluene-in-Hexane reference standard for resolving spectral bandwidth of UV/VIS 810.53
spectrophotometers

Assessing the photometric and wavelength performance of UV/VIS spectrophotometers is a necessary part of
keeping your laboratory in accordance with GLP, European Pharmacopeia (UP) or United States Pharmacopeia
(USP) standards.

The spectral bandwidth (SBW) refers to the physical slit-width of the spectrophotometer's monochromator, which
has a direct impact on the instrument's resolution. In turn, spectral resolution is the ability to resolve two
neighbouring peaks. Reconciling the resolution is an integral component in getting accurate wavelength and
photometric readings on your spectrophotometer.

Recognizing the need for periodic assessment of SBW and pursuant to EP and USP requirements, FireflySci has
expanded its range of calibration products to include this Toluene in Hexane standard.

Delivery includes:

 High-purity solution of 0.02% Toluene in Hexane
 High-purity Hexane blank

Suggested use:

1. The EP recommends comparing the peak maximum at 269nm with the peak minimum at 266nm.
2. Determine the resolution by running the Toluene in Hexane against the Hexane blank between 260 and

270nm and calculate the peak minima and maxima.
3. Finally, calculate the peak-to-trough ratios and compare the results with the instruments reference

values. If they are considerable lower, you may wish to contact your instrument manufacturer.

Please note that, in accordance with NIST guidelines, this standard must be recalibrated annually.

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All prices are shown in Euro and are subject to VAT where applicable.