Page 48 - Live-cellanalysis handbook

Live-Cell Analysis Handbook — Third Edition

Chemotaxis assay

Taylor, et.al., used the IncuCyte® chemotaxis assay to study netrin-1, a cellular guidance signal. The authors demonstrated that netrin-1
inhibits human monocyte and murine macrophage migration towards C5a, a complement peptide with potent chemotactic and
anaphylatoxic properties that upregulate endothelial adhesion molecules, increase vascular permeability, and localize leukocytes and
inflammatory molecules at sites of infection.

Netrin-1 reduces monocyte and macrophage chemotaxis towards the complement component C5a. Taylor, L., Brodermann, M. H.,McCaffary, D., Iqbal, A.
J., Greaves, D. R. PLoS ONE, 2016 Aug 10; 11(8).

A study by Pasqualon, et. al., demonstrated that syndecan-1, a surface-expressed proteoglycan on epithelial tumor cells, promotes
macrophage migration inhibitory factor (MIF) binding and MIF-mediated cell migration. Invasion studies showed that MIF induced the
chemotactic migration of A549 tumor cells, wound closure and invasion into Matrigel without affecting cell proliferation. These MIF-
induced responses were abrogated by silencing of syndecan-1. The authors conclude that this may represent a relevant mechanism
through which MIF enhances tumor cell motility and metastasis.

Cell surface syndecan-1 contributes to binding and function of macrophage migration inhibitory factor (MIF) on epithelial tumor cells. Pasqualon, T.,
Lue, H., Groening, S., Pruessmeyer, J., Jahr, H., Denecke, B., Bernhagen, J., Ludwig,A. BBA-Mol Cell Res. 2016 Apr; 1864 (4).
Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesion. Bulanova, D; Akimov, Y; Rokka, A; Laajala, T;
Aittokallio, T; Kouvonen, P; Pellinen, T; Kuznetsov, S. Cell Adhesion & Migration. 2016 Oct.
Potent EMT and CSC phenotypes are induced by oncostatin-M in pancreatic cancer. Smigiel, J; Parameswaran, N; Jackson, MMol Can Res. 2017 Jan.

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