Page 470 - Veterinary Immunology, 10th Edition

VetBooks.ir

FIG. 15.24 A schematic diagram showing the method of
production of monoclonal antibodies. Antibody-producing plasma
cells are fused with myeloma cells. The resulting hybridoma cells
are cultured, cloned, and selected so that they produce antibodies
against the antigen of interest.

There are three pathways by which cells can synthesize

nucleotides and therefore nucleic acids. The myeloma cells are
selected so that they lack two enzymes: hypoxanthine
phosphoribosyl transferase and thymidine kinase. As a result, they
cannot use either thymidine or hypoxanthine and are obliged to use
an alternative biosynthetic pathway to convert uridine to

nucleotides. The fused cell mixture is therefore grown in a culture

470