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VSP 2018 Operations Manual



                                                4. Place sheets of plastic wrap over the toilet bowl, leaving a
                                                   slight dip in the center.
                                                5. Put the toilet seat down.
                                                6. Pass some stool onto the plastic wrap. Do not let urine or
                                                   water touch the stool specimen, if possible.
                                                7. Using the spoon given to you, place bloody, slimy, or
                                                   whitish areas of the stool into the container first. Fill the
                                                   cup at least 2/3 full, if possible.
                                                8. Tighten the cap.
                                                9. Wash your hands.
                                                10. Label the specimen jar with your name, the date, and your
                                                   cabin number.

                              13.4.5.2.2    Medical Staff Instructions
                                            Specimen Labeling
                                            Please ensure that each specimen is properly labeled with the
                                            following:
                                                •  Date of collection.
                                                •  Unique identifying number. (A separate log containing
                                                   unique identifying number linked to case name must be
                                                   kept on the ship.)
                                                •  Notation on use of antidiarrheal or antibiotic medication.

                                            Collection, Storage, and Transport
                                            Complete guidelines for collection and storage of specimens for
                                            viral, bacterial, and parasite analysis are listed below, although it
                                            may not be necessary to implement all procedures during each
                                            investigation. Transport of specimens will be arranged in
                                            collaboration with VSP.

                          13.4.5.3  Guidelines for Collecting Fecal Specimens for Viral Diagnosis
                                     (Modified from MMWR, 1990; 39[RR-5];19.)

                              13.4.5.3.1    Stool for Viral Diagnosis
                                                1. Collect stool specimens in the first 48 hours. Specimen
                                                   collection should not await the results of epidemiologic and
                                                   other investigations because delay will almost certainly
                                                   preclude a viral diagnosis. If information gathered
                                                   subsequently indicates that a viral etiology is unlikely, the
                                                   specimens can be discarded.

                                                2. Collect 10 diarrhea bulk specimens, if possible. Bulk
                                                   specimens, enough to fill a large stool cup, are preferred.
                                                   Serial specimens from persons with acute, frequent, high-
                                                   volume diarrhea are particularly useful. The smaller the
                                                   specimen and the more formed the stool, the lower the



                                Annex 13.4: Acute Gastroenteritis Outbreak Investigation; 180
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