Page 53 - Phytochemistry -1 (PG404) / Clinical Pharmacy 2nd level students ( 2019 )
P. 53
Clinical pharmacy PharmD program Third level Phytochemistry-1 (PG-504)
Bitter principle
• Natural compounds composed of C, H, O but not N, they don´t have
a definite common structure but they are all bitter in taste.
EX: Khellin in Ammivisnaga
OCH 3
O O CH 3
OCH O
3
Chemical test:
Add few NaOH / KOH pellets to a drop of water extract of Ammi visnaga
(Khellin extract) → rose red color is developed.
✓ Khellin is a chromone bitter principle present in Ammi visnaga
fruits in addition to visnagin, khellol glucoside, fixed oil and
protein.
✓ Used as a smooth muscle relaxant (ease passage of renal calculi),
in bronchial asthma, angina, antispasmodic for renal & bilary
duct.
✓
A micro method for estimation of khellin in Ammi visnaga fruits
Principle
Preparative isolation of khellin then measuring the A or %
transmittance at UV 420 nm after the development of yellow color
with 2N H2SO4
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