Page 91 - Practical Technology 2025

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Clinical Pharmacy-Pharm D Level Three 2 Semester 2024/2025 Pharmaceutical Technology (PT 607)

Lab (12): Microscopy
Microscope is an instrument that enlarge small objects and make them visible.

Classification

Automatic and
Image Analysis
Optical microscopy Electron microscopy
Microscopes

1-150µm 0.001µm

Manual Optical Microscopy -Transmission Microscopy
1. -Scanning Electron
Microscoy

Properties of an ideal microscope :

If microscope cannot be able to separate two objects that are closely situated, we
’
don t able to get enough information, hence it is called empty magnification.
1. Resolution: the capacity of an optical instrument to distinguish or
separate two adjacent objects.

2. Resolving Power (limit of resolution): the minimum distance existing
between two objects when these objects can still be observed as separate

objects
d= (0.61 x λ)/ NA

1. Optical microscopy (1-150µm)

✓ Optical microscopy is a technique employed to closely view a sample
through the magnification of a lens with visible light.
✓ According to the optical microscopic method, an emulsion or suspension

is mounted on ruled slide on a mechanical stage.

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