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TRACK 1 Technical Program

nanoplatform with well-aligned radiotherapeutic and diagnostic properties 12:40pm Albumin-Biomineralized Gd:CuS Theranostic Nano-
for better integration into standard radiation therapy practice, we developed probes for In Vivo Photoacoustic /MR Imaging-Guided Tumor
a gold- and superparamagnetic iron oxide nanoparticle (SPION)-loaded Photothermal Therapy
micelle (GSM). Intravenous injection of GSMs into tumor-bearing mice led
to selective, tumoral accumulation, enabling magnetic resonance (MR) Technical Presentation. NEMB2016-6096
imaging of tumor margins. Subsequent irradiation led to a 90-day survival
of 71% in GSM-treated mice, compared with 29% for irradiation-only mice.
Furthermore, measurements of the GSM-enhanced MR contrast were highly Weitao Yang, Jin Chang, Tianjin University, Tianjin, China, Bingbo
predictive of tumor response. Therefore, incorporating GSMs with radiation Zhang, Tongji University, Shanghai, China
therapy could augment cancer treatment by facilitating imaging, increasing
the efficacy of therapy, and helping to predict response. Moreover, since Photothermal therapy (PTT) has attracted increased interests due to its
GSMs are prepared using a highly modular synthetic pathway, additional noninvasiveness and low systemic adverse effects. There is, however, still a
components, including standard and alternative therapeutics could readily strong desire for biocompatible and versatile PTT agents with accurate im-
be incorporated into the micelle’s core while targeting moieties (e.g. tu- aging guidance on its therapy efficiency. In this study, bovine serum albumin
mor-specific antibodies or Fab’s) can be coupled onto the unobstructed mi- (BSA), as a biotemplate was employed for biomimetic synthesis of Gd-in-
celle surface, further broadening the range and types of tumors that can be tegrated CuS hybrid nanoprobes (Gd: CuS CuS CuS CuS CuS CuS@BSA
effectively treated. The extravasation properties of these particles may also promising for imaging guidance on potent tumor ablation with high spatial
make them useful in treating diseases localized to regional lymph nodes, resolution and deep tissue penetration.
such as Hodgkin’s lymphoma. Therefore, it is envisioned that translation of
GSM to oncology could have far reaching implications.
1-3
Despite the perceived biocompatibility of gold nanoparticles, the protracted ADVANCED OPTICAL NANOPARTICLES AND IMAGING
elimination and long-term persistence of gold within many organ systems
remains a concern for clinical translation. To improve the excretion of gold in TECHNIQUES
our nanoplatform, we prepared micelles with 0.9 nm or 5 nm gold nanopar-
ticles tightly packed within the hydrophobic core. The use of many small Sam Houston 4:00pm - 5:30pm
gold nanoparticles was expected to allow for improved excretion of gold,
compared with single large gold nanoparticles, owing to the smaller size
and larger surface-to-volume ratio of the individual gold nanoparticles within Session Organizer: Andrew Tsourkas, University of Pennsylvania,
the micelle. Following intravenous administration of micelles, organs were Philadelphia, PA, United States
harvested and examined for gold content using inductively coupled plasma
optical emission spectrometry (ICP-OES) for up to 3 months post-injection. 4:00pm Plasmonic Nanoparticle Vesicles for Cancer Imaging
The micelles containing 0.9 nm gold nanoparticles showed a 72% and 67% and Therapy
reduction in gold content in the liver and spleen, respectively, between 1
day and 3 months post-injection, compared with a 38% and 35% reduction Keynote. NEMB2016-5930
in mice receiving micelles with 5 nm gold nanoparticles. Furthermore, feces
and urine analysis revealed approximately 7.5 and 100 times more gold, re-
spectively, in mice that received micelles with 0.9 nm gold nanoparticles one Shawn Chen, National Institute of Biomedical Imaging and Bioengi-
day after injection. These findings suggest that the excretion profile of inor- neering, Bethesda, MD, United States
ganic nanomaterials may be improved if clusters of small inorganic materials
are used in favor of single solid particles. Vesicular structures can have tailored structural properties and built-in
releasing mechanisms, controlled by stimuli-responsive polymer building
12:20pm Magnet-Optical nanoparticles for Magnetomotive Pho- blocks towards cancer diagnosis and therapy. In this talk, examples of how
toacoustic Imaging gold nanoparticles are assembled to have enhanced optical properties
(scattering, photothermal conversion, and SERS) for Raman detection, pho-
toacoutsic imaging and photothermal treatment of cancer will be illustrated.
Technical Presentation. NEMB2016-6145 The interparticle coupling and disintegration of the plasmonic vesicles can
be tailored by altering structural parameters and conformational changes of
Xiaohu Gao, Junwei Li, University of Washington, Seattle, WA, the covalently bound polymer brushes.
United States
4:30pm Deep and high-resolution three-dimensional tracking of
Photoacoustic imaging has emerged as a highly promising tool to diagnose single particles using nonlinear and multiplexed illumination
tumor lesion site with deep tissue penetration. However, image contrast
under in vivo conditions is far from optimal due to background signals from Technical Presentation. NEMB2016-6003
tissue. We have previously developed the new concept of magnetomotive
photoacoustic (mmPA) imaging, which is capable of dramatically reducing
the influence of background signals and producing high-contrast images. Evan Perillo, Yen-Liang Liu, Cong Liu, Andrew K Dunn, Tim Yeh,
Here we addressed two significant advances toward clinical translation of University of Texas at Austin, Austin, TX, United States
this technology. First, we prepared a new class of compact, uniform, mag-
neto-optically coupled core-shell nanoparticles through localized copoly- Molecular trafficking within engineered three-dimensional multicellular models
merization of conducting polymers on an iron oxide nanoparticle surface. is critical to the understanding of the development and treatment of various
The resulting multifunctional nanoparticles solve the photo-instability and diseases including cancer. However, current tracking methods are either con-
small-scale synthesis problems previously encountered by the gold coating fined to two dimensions or limited to an interrogation depth of 15 µm. Here
approach. In parallel, we developed a new generation of mmPA imaging we present a new 3D tracking method capable of quantifying rapid molecular
featuring cyclic magnetic motion and ultras! ound speckle tracking (USST), transport dynamics in highly scattering environments at depths up to 200 µm.
whose imaging capture frame rate is several hundred times faster than the The system has a response time of 1 ms with a temporal resolution down to
photoacoustic speckle tracking (PAST) method we demonstrated previously. 50 µs in high signal-to-noise conditions, and a spatial localization precision as
These advances enable robust artifact elimination caused by physiologic good as 35 nm. Built upon spatiotemporally multiplexed two-photon excitation,
motions and first application of the mmPA technology in vivo for sensitive this approach requires only one detector for 3D particle tracking and allows 17
solid tumor imaging. for two-photon, multi-color imaging. 3D tracking of epidermal growth factor
receptor (EGFR) complexes at a depth of 100 µm in tumor spheroids is demon-