Page 23 - An Identity Crisis

Pre-Lab

Solutions

DNA Background Procedure

1. What is the key difference between 6. How much DNA goes into each lane?
PCR and RFLP?
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7. What always goes in the first lane of the gel?
2. Why does this difference make PCR better ...............................................................................
suited for forensics?
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8. Why is it important to write down the loading
order of the samples in the wells?
3. What causes DNA to move during electrophoresis?
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9. Which direction does DNA travel? Should
4. How is the DNA sorted after electrophoresis? the wells be on the end near the black or red
electrode?
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5. What is a ladder?

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THE MYSTERY OF LYLE AND LOUISE 23