Page 24 - An Identity Crisis

Lab

Procedure

PREPARING THE GEL (45 MIN) completely cover the gel. Ensure that the wells
are filled.
1. Remove the cap from the bottle of agarose gel
and microwave in 15-30 second intervals until 10. Carefully remove the comb.
the agarose has completely melted. Ensure
that the gel does not boil over the bottle. Allow
agarose cool to at least 60°C before continuing. PIPETTING ORDER

2. Shake the bottle of BlueVis Gel Stain well. GEL A GEL B
3. Transfer 4 mL of BlueVis Gel Stain into the Ladder Ladder
bottle containing agarose. Recap the bottle Lyle Mondelo Adult Woman (Car)
and swirl to thoroughly incorporate the stain. Louise Mondelo Female Child (Car)
4. Position the gel box on a level surface and Wally Mondelo Male Child (Car)
insert the gel tray, ensuring that all sides are Jan Mondelo Adult Woman (Cabin)
tightly sealed. John Wayne Gretzky Adult Male (Cabin)
Firewood (Cabin)
5. Place the comb at one end of the gel tray.
Fireplace (Cabin)
6. Slowly pour the agarose into the gel tray until
the central cavity and the crevices between
each tooth in the comb are filled. PREPARING THE SAMPLES
The samples will be divided over two gels, A and B,
7. Allow the gel solidify and turn slightly opaque to keep the knowns separate from the unknowns/
(approximately 20—30 minutes).
evidence. Both Gels A and B will be run in triplicate,
8. Remove and turn the gel tray so that the wells with three groups running a copy of gel A and three
are located near the black (-) electrode and groups running a copy of gel B.
the gel points toward the red (+) electrode.

9. Fill the gel box with enough 1x BlueVis Gel A will contain samples that are known; DNA
buffer solution provided by your teacher to that should be run on Gel A will have blue caps. Gel
B contains unknown samples from the cabin and

24 THE MYSTERY OF LYLE AND LOUISE