Page 25 - An Identity Crisis

car accident; these samples will have red caps. The with three groups running a copy of gel A and three
ladder contains five lengths of DNA: 150, 300, 500, groups running a copy of gel B.
650, and 1000 base pairs (bp). The ladder is labeled
in black and will be run in the first lane of both gels. 1. Before pipetting each sample place a new tip
onto the pipette.
1. Collect a set of samples for the gel you will be
running and set them before you. 2. Ensure pipette is set to 10 μL.
3. Tap the sample tube against the palm of your
2. Order your samples according to their pipetting
order. hand to settle the contents at the bottom.
4. Open the sample tube.

LOADING THE GEL 5. Press down on the pipette plunger until
The samples will be divided over two gels, A and B, you reach the first stop. The plunger will
to keep the knowns separate from the unknowns/ depress. further, but a distinct change in
evidence. Both Gels A and B will be run in triplicate, resistanceshould be noticeable.

THE MYSTERY OF LYLE AND LOUISE 25