Page 51 - Адууны ям өвчний тандалт, үүсгэгчийн молекул биологийн судалгаа

1. To collect serum samples and swabs from the target aimags and to test serum

samples by CFT;
2. To isolate pure cultures from swabs;

3. To make differential diagnosis of causative agent by using standard bacteriological
method;

4. To isolate bacterial genomic DNA and confirm by PCR;
5. To determine and compare nucleic acid sequence of specific gene of both local and

standard strains.

Results

In order to determine prevalence rate of equine glanders, 520 horse blood serum
samples from western and central regions of Mongolia and 493 horse blood serum

samples from Baganuur, Nalaikh and Khan-Uul districts were checked by CFT in 2014
and 2015 respectively for surveillance. As a result, 24 of 520 samples collected in 2014

were positive or prevalence rate is 4.6%, whereas 65 of 493 samples taken in 2015 were

positive or prevalence rate is 11.56%.
As well, inoculation from swabs to both simple and selective nutrient media was

0
made by using Koch dilution method and they were incubated at 37 С for 24 to 72 hours.
Meat peptone agar (MPA) containing glycerol and blood agar nutrient media were used
for above purpose. Colonies, which are morphologically similar to bacterium B.mallei,

were collected and pure cultures were isolated. Biochemical activity, antibiotics
sensitivity and motility of cultures were measured and diagnosed differentially. Local

strains sent by us were resistant to two antibiotics Colistin and Polymixin B, non-motile
and oxidase positive strains. Then these strains were cultivated in liquid nutrient

medium, genomic DNA was isolated as described in the methodology, and specific gene

stated in OIE recommendation was amplified and confirmed by PCR. Nucleotide
sequences of these genes were determined and compared to sequences of the gene of

International Genebank, and finally it was identified the bacterium is causative agent of
equine glanders at genetic level.

Conclusion

1. Prevalence rate of equine glanders was 4.61% in western and central aimags of

Mongolia in 2014, while 11.1% in areas around UB city in 2015.

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