Page 46 - Бог малын сохор догол өвчнийг оношлох ФХЭБУ-ын эсрэгтөрөгч бэлтгэн сорьсон дүн

Бог малын сохор догол өвчнийг оношлох ФХЭБУ-ын эсрэгтөрөгч бэлтгэн сорьсон дүн

2. To identify the causative agents of contagious agalactiae of small
ruminants by cultural, morphological and biochemical properties and

characterization by PCR
3. To prepare a protein antigen and positive control serum for ELISA and

evaluate it’s sensitivity and specificity

In this study collected and used for bacteriological examination from milk and
yey swabs from 191 infected animals. Also, we collected 344 sera samples

of goat and sheep in Khentii and Uvur-Khangai province and used for

evaluation of an antigen, developed by us.
During this study we had been isolated 37 mycoplasma like agents form

above mentioned samples and had been characterized as the M.agalactiae by
PCR.

All isolates were positive by standart PCR with M.agalactiae specific
primers, developed by previously foreign researchers and recommended by

OIE for identification of causative agents of this infection.

Also, we had performed the partial sequencing of the UrvC gene of
isolates and compared with reference strains. Our results suggested that, all

Mongolian isolates are belongs to group similar to M.agalctiae PG2
(accession number CU179680) and genetically different from M.agalctiae

5632 (accession number FP671138).
From above mentioned 37 isolates, we had selected 6 isolates, which

were determined by PCR as the M.agalactiaethese selected 6 of them to use

for preparation of a protein antigen.
For the extraction of protein antigen from M.agalactiae isolates were used

0.5% n-Luarelsarcosine. Extracted protein antigen has high specificity, but it’s

sensitivity were comparatively low than commercial ELISA kit.

Гомбосүрэнгийн Өлзийсайхан 43