Page 47 - Гахай, тахианы микоплазмозын оношлогоо

Гахай, тахианы микоплазмозын оношлогоо

laying hen resulted in 5 to 10% mortality rate of all embryos and dropped egg

production rate by 10 to 20%. Of chickens infected by bacterium M. synoviae,
decrease of egg production rate and loss of survival rate accounted for 5 to 10% and

5 to 7% respectively, and more than 5% of chicks are dead [25]. Significant decrease
of egg production rate is observed 4 weeks after bacterial infection and then egg

nutritional value drops [6]. Because causative agent spreads via airborne droplets,
mass culling of hens in the infected farm makes the disease the most economic loss

causing infection [7].

Due to deaths of chicken caused by M. gallisepticum infection during late 1970 in
USA, more than 118 million USD worth loss was caused to poultry farmers and

consumers [8]. Infection of bacterium Mycoplasma gallisepticum in turkey farms of
North Carolina, USA led to a total of 2.5 million USD worth loss for the farmers [9].

Except that avian and swine mycoplasmoses were diagnosed first in our country in
1965 and 2006 respectively, any other surveillance of the diseases were not

performed. Increase of the numbers of pig and poultry farmers in the last years

necessitated the surveillance of this disease and prevention of economic losses and
risks.

Therefore, we considered surveillance and diagnosis of the disease in both pig and
poultry farms are essential for prevention and control measures in the future, and

performed this study.

Goals and objectives of the study

The goal of the study is to diagnose swine and poultry mycoplasmoses by use of

serological, bacteriological and molecular biological techniques. To achieve above
goals the following objectives were accomplished:

-To collect samples from swine and poultry farms;

-To perform serological testing in blood serum of swine and chickens and detect
antibody produced against M. hyopneumoniae, M. synoviae and M. gallisepticim;

-To isolate culture of causative agent by bacteriological testing;

-To confirms the culture by PCR technique.

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