Page 50 - Гахай, тахианы микоплазмозын оношлогоо

Гахай, тахианы микоплазмозын оношлогоо

color of liquid nutrient medium of which changed and no specific band was shown at

649 bp length for all samples.

PCR by DNA extraction from 40 chicken cultures demonstrated 20 samples show

specific band similar to positive control of M. gallisepticum at 185 bp nucleotide pair.
Eight of the former samples give specific band at 207 bp nucleotide pairs similar to

positive control of M. synoviae.

CONCLUSIONS

1. According to the study for diagnosing swine enzootic pneumonia, specific antibody

was detected by ELISA testing in 13.7% of 581 serum samples tested and detection
of specific antibody against M. hyopneumoniae, although which was not detected by

both bacteriological PCR testing, demonstrated the disease is prevalent at a certain
rate in pig farms.

2. Specific antibodies against M. gallisepticum and M. synoviae were detected in
27.4% and 46.5% respectively of a total of 456 serum samples of chickens and it was

confirmed that the cultures isolated from chicken samples were identified to be M.

gallisepticum and M. synoviae by bacteriological and PCR testing.

3. Separate detection of causative agents of chicken mycoplasmosis M. gallisepticum

and M. synoviae in some chicken farms of our country and detection of both agents
in single sample reveals the farms are infected with causative agents of the disease.

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