Page 49 - Гахай, тахианы микоплазмозын оношлогоо

Гахай, тахианы микоплазмозын оношлогоо

RESULTS

Results of serological testing: Of 581 swine blood serum samples, 12 samples

were suspicious, whereas specific antibody to M. hyopneumoniae was detected in 80

samples. As well specific antibody was detected in 6.9 to 20.5% of swine in some
farms of Uvurkhangai, Bulgan and Orkhon aimags of Khangai region and 10% of

swine in Darkhan-Uul and Tuv aimags of central region (table 2).

Specific antibodies against M. gallisepticum and M. synoviae were detected in 27.4%

and 46.5% of blood serum samples respectively of 46 chickens tested by ELISA.

Specific antibody to M. synoviae was detected in 10.5 to 30% of chickens of farms
and families and antibody to M. gallisepticum detected in 8% in Zavkhan, Govi-Altai

and Uvs aimags of western region, whereas specific antibodies to M. gallisepticum
and M. synoviae were detected in 56 to 58% and 9 to 74% of chickens of some farms

and families of Uvurkhangai, Arkhangai, Bayankhongor, Bulgan and Orkhon aimags
of khangai region. Specific antibody to M. synoviae was detected in 0.2 to 72% of

chickens of some farms in Darkhan-Uul and Tuv aimags of central region, while

antibodies to M. synoviae and M. gallisepticum were detected in 60% and 40%
respectively of chickens of some farms and families in Dornod aimag of eastern

region. It was demonstrated that specific antibodies to both M. gallisepticum and M.

synoviae were detected in 7.6% and 48.4% respectively of chickens of some poultry
farms of Ulaanbaatar city (table 3).

Results of bacteriological testing: As a result of inoculations from 172 swine and
218 chicken samples made in PPLO nutrient medium, followed by incubation at 37˚С

for 48-96 hours, color of nutrient medium changed for 70 swine samples and 40
chicken samples. Dilution of broth cultures at 1:1000 to 1:100000, spreading of 200

µl aliquots on the surface of selective mycoplasma agar and CO2 incubation at 37˚С

for 48 to 96 h did not result in growth of mycoplasma like colonies from swine
samples, while it resulted in growth of mycoplasma like colonies from 28 chicken

samples.

Results of molecular biological testing: DNA was purified by use of phenol,

chloroform and Sigma-Aldrich DNA extraction kit from cultures of 70 swine samples,

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