Page 7 - exemple
P. 7

B- Fehling test for reducing sugars


           Pour 10 ml of water in a test tube A, and 10 ml of a glucose
           solution in a test tube B. Add to each tube 2 ml of the blue
           colored Fehling solution.
           Heat the mixture in tubes A and B.
           In tube A, the blue coloration persists whereas a brick red
           precipitate appears in tube B indicating the presence of a
           reducing sugar. Fehling test gives a positive result with all
           the monosaccharides and disaccharides except sucrose.


                                                                             Doc 3- Identification of reducing
                                                                                          sugar.

               3       Iodine water test for starch (performed at cold temperature)


           Pour few drops of iodine water (brown orange) in a test tube
           containing a solution of cooked starch. A dark blue
           coloration appears. We say that the reagent of starch is iodine
           water.
           Starch is found in potatoes, rice, wheat…
           Starch is a carbohydrate without a sweet taste. It is soluble in
           hot water only. Its molecule is formed by an association of
           many glucose molecules.
           By performing the iodine water test directly on a piece of
           bread or on a slice of potato or banana, a dark blue
           coloration appears indicating the presence of starch
           (Doc. 4).                                                         Doc 4- Iodine water test on a piece
                                                                                         of bread.



               4       Identification test for lipids



           Place or scrub a foodstuff rich in lipids on a paper; a
           translucent spot appears.
           Wait for an hour, we observe that the spot becomes larger
           and doesn’t disappear when heated.
           Oil and milk creme have the same properties. They are lipids.




                                                                                 Doc 5- Only lipids leave a
                                                                                translucent spot on a paper.




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