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Klingeborn et al. Page 47
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A summary of the most relevant characteristics of common exosome and small EV isolation methods.
Preferred analysis † EM, NTA, WB 2 nd isolation method required EM, WB, NTA, MS, NGS, LA WB, MS, NGS, LA EM, NTA, WB, MS, NGS, LA
Table 1 * Biological activity is impacted by different isolation methods by altering exosome integrity. Examples of biological activity includes exosome binding and uptake, enzyme activity, and other protein and † Analyses that the resulting EV preparation is most suited/optimal for based on purity and physical integrity of the exosomes and small EVs. DUC, differential ultracentrifugation; PEG, polyethylene glycol precipitation; DGUC, density gradie
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Biological activity * ++ + ++ + +++
Cost Low Low - High Low Medium to High Medium
Ease of use ++ +++ + +/++ ++ blotting; MS, mass spectrometry; NGS, next-generation sequencing; LA, lipidomic analysis.
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Exo purity ++ + +++ ++/+++ ++ nucleic acid-induced activity measured in target cells.
Yield ++ +++ + ++ +/++
Method DUC PEG DGUC IAC SEC
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Prog Retin Eye Res. Author manuscript; available in PMC 2018 July 01.
