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Klingeborn et al. Page 43
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Figure 4. Comparison of myocilin-associated exosomes in fresh aqueous humor samples from
normal and glaucoma patients undergoing surgery
Shown are representative Western blots of volume-equivalent samples taken from aqueous
humor subjected to differential centrifugation steps. Pre-spin shows whole aqueous humor;
cell debris shows resuspended pellet of whole aqueous humor subjected to 10,000 g;
supernatant from pre-spin was subjected to centrifugation at 100,000 g giving exosomes in
pellet and soluble proteins (sol. Prot.) in supernatant (Perkumas et al., 2007). Permission
pending.
Author Manuscript
Author Manuscript
Author Manuscript
Prog Retin Eye Res. Author manuscript; available in PMC 2018 July 01.
