Page 63 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 03 Page 1 of 3

3. Estimation of Crude Protein- Macro

Kjeldhal Distillation

FVolumetric Pipette, 25 ml, 20 ml, 10 ml (Picture-6)
SCOPE
To estimate the crude protein in feed feedstuffs by FCylinder, graduated, 500 ml, 1000 ml (Picture-7)
Macro Kjeldhal distillation method.
FConical flask, 250 ml, 500 ml (Picture-8)
FReagent bottle, 1000 ml, 500 ml, 250 ml (Picture-
PRINCIPLE
FSample is digested in sulphuric acid using copper 9)
sulphate as catalyst and by adding sodium
FHot Air Oven pre calibrated (Picture-10)
sulphate anhydrous to elevate the boiling point
converting organically bound nitrogen to FNi - Spatula.
ammonium sulphate when heated with excess
alkali, ammonia is liberated which is distilled into
known excess of saturated acid.
FThe un reacted (un neutralized) acid is back titrated
with standard alkali.

FFrom titrated values the nitrogen content and crude
protein are calculated by multiplying by 6.25
(100/16). Picture-1 Picture-2

APPARATUS
FAnalytical Balance, 1mg / 0.1mg
accuracy (Picture-1)
FKjeldhal flask, 500 / 800 ml (Picture-2)

FDigestion chamber (Picture-3)

FMacro Kjeldhal distillation assembly (Picture-4)

FBurette, 25 / 50 ml (Picture-5) Picture-3 Picture-4

Prepared by : Approved by :
51 Director - Technical