Page 90 - Feed Lab

Estimation of Pepsin Digestibility Suguna Management System Ver 1.0 / SOP / FQL / P2 - 15 Page 2 of 2

every 30 minutes at 42-45° C temperature.
( Picture - 12)
FAfter the completion of 16-20 hours, take the flask
out of the incubator and filter through Qualigens
filter paper 615A or Whatmann filter paper No.1.
(Picture - 13)

FWash the residue with 200 to 250 ml of distilled
water and dry in hot air oven for 30 minutes. Above
Picture - 9 Picture - 10
dried residue with filter paper is transferred into the
digestion tube for protein digestion. (Picture - 14)
REAGENTS
FPepsin (activity1:10,000)
FDo the digestion and distillation as per the crude
F0.075N Hydrochloric acid protein estimation. The arrived protein value is
called percentage of pepsin indigestible protein.
FPotassium sulphate, AR grade
Do the blank estimation with filter paper and without
FCupric Sulphate, AR grade sample.

FNitrogen Free concentrated Sulphuric acid, AR
Grade
F40 % Sodium hydroxide w/v, AR grade

F0.2 N Hydrochloric acid - 17.46 ml/L of distilled
water, AR grade 35.4 % assay

FPotassium Hydrogen Phthalate, AR grade
F4 % Neutral Boric Acid (BA)
Picture - 11 Picture - 12
PROCEDURE
FTake 0.5g of ground (1mmsize) sample in a 250ml
flat bottom flask and add 0.5 g of pepsin. Add 50 ml
of 0.075NHcl to the above flat bottom flask and
stopper. (Picture - 11)

FShake well and keep the flask in incubator for 16-
20 hours with occasional shaking or shake for
Picture - 13 Picture - 14

CALCULATION
TV - B X N. of Hcl X 1.401
Pepsin Indigestibility Protein % = -----------------------------------------
Sample Weight, g

Crude protein - pepsin indigestible protein
Pepsin digestibility % = ------------------------------------------------------ X100
Crude protein

Prepared by : Approved by :
78 Director - Technical