Page 86 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 13 Page 1 of 2

13. Estimation of Urease Activity (PH Difference)

SCOPE
To estimate the urease activity for soya bean meal by
pH difference method.

PRINCIPLE
FAnalytical Balance, 1 mg / 0.1 mg accuracy
(Picture - 1)
FWater bath capable of maintaining temperature of Picture - 3 Picture - 4
o
o
20 C-30 C. (Picture - 2)
FpH - meter fitted with glass and calomel
electrode and equipped to test 25 ml of
solution. (Picture - 3)

FVolumetric flask, 100 ml, 500 ml, 1000 ml.
(Picture - 4)

FHot Air Oven pre calibrated (Picture - 5)

Picture - 5 Picture - 6

REAGENTS
FPhosphate Buffer Solution(PBS)

F Dissolve 3.403 g of Potassium dihydrogen
phosphate, AR grade in 100 ml of distilled water.

F Dissolve 4.355g of dipotassium hydrogen
Picture - 1 Picture - 2 phosphate, AR gradein100mlofdistilledwater.

F Combine the two solutions and make up to 1000
ml. If reagent is pure, the pH should be 6.96

Prepared by : Approved by :
74 Director - Technical